What are all the ways to increase rotten charges in all of the Rotten Blood spawns?

Question

While gaining access to the bosses in Rotten Blood, what are all the ways a players can increase their rotten charges in each of the spawns?

Answer 1

There is 4 spawns with 4 bosses. On each spawn you get charges by killing monsters and every spawn have also extra mechanic to make your charges increase. Mechanic is almost similar on every spawn:

Jaded Roots - leads to the boss Murcion

• Using  Organic Acid on Small Spore Reservoirs for 1 Rotten Charge (-20 seconds for the boss access) 
• Using a Spore Reservoir (white dot on the map) for 15 Rotten Charges (-5 minutes for the boss access) 

Putrefactory - leads to the boss Ichgahal

• Using Lichen Gobbler on specific tiles in the lower floor of the hunting area for 1 Rotten Charge (-20 seconds for the boss access)

• Using a Large Spore Reservoir (white dot on the map) for 15 Rotten Charges (-5 minutes for the boss access)

 

Gloom Pillars -  leads to the boss Chagorz

• Using Darklight Obsidian Axe on Basalt Pillar. Each use give you 3 charges.
• Using a Darklight Obelisk (white dot on the map) for 15 Rotten Charges (-5 minutes for the boss access)

Darklight Core - leads to the boss Vemiath

• Using  Unstable Darklight MatterorYellow Darklight Matter  on Darklight Pore.The life of the yellow version will give 3 points, the unstable version 1 point.
• . 
• Using an Darklight Obelisk(white dot on the map) for 15 Rotten Charges (-5 minutes for the boss access)

Source: Tibiopedia - link